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Effects of Enamel Matrix Derivative on Proliferation/Viability, Migration, and Expression of Angiogenic Factor and Adhesion Molecules in Endothelial Cells In Vitro
Author(s) -
Bertl Kristina,
An Na,
Bruckmann Corinna,
Dard Michel,
Andrukhov Oleh,
Matejka Michael,
RauschFan Xiaohui
Publication year - 2009
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2009.090157
Subject(s) - enamel matrix derivative , in vitro , microbiology and biotechnology , adhesion , chemistry , matrix (chemical analysis) , cell adhesion molecule , matrix metalloproteinase , biology , biochemistry , regeneration (biology) , organic chemistry , chromatography
Background: The aim of this study was to test in vitro the effect of enamel matrix derivative (EMD) on the proliferation/viability, migration, and expression of angiogenic factor and adhesion molecules in human umbilical vein endothelial cells (HUVECs). To date, discussions on angiogenic effects of EMD are rather controversial. Methods: The effect of EMD on the proliferation/viability of HUVECs after 24 hours was measured using 3,4,5‐dimethylthiazol‐2‐yl‐2,5‐diphenyl tetrazolium bromide (MTT) assay and direct cell counting. Cell migration was observed in an especially adapted in vitro monolayer wound‐healing model. The expression of angiogenic factor angiopoietin‐2 (ang‐2) and adhesion molecules intercellular adhesion molecule (ICAM)‐1 and vascular endothelium‐selectin (E‐selectin) was quantified with real‐time polymerase chain reaction (PCR). Results: The proliferation/viability of HUVECs measured in MTT assay was stimulated by 0.1 μg/ml EMD and inhibited by higher doses (50 to 100 μg/ml), but the total number of cells was not affected. Cell migration in the wound‐healing assay was promoted by EMD at doses of 0.1 to 50 μg/ml and inhibited at 100 μg/ml. The highest expression level of all three tested genes (ICAM‐1, E‐selectin, and ang‐2) was observed at 50 μg/ml EMD. Conclusion: The results of the present in vitro study show the potential influence of EMD on the angiogenic activity of HUVECs, which may play an important role in periodontal tissue regeneration and wound healing.