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Treatment of Experimental Periodontal Disease by Photodynamic Therapy in Rats With Diabetes
Author(s) -
de Almeida Juliano Milanezi,
Theodoro Leticia Helena,
Bosco Alvaro Francisco,
Nagata Maria José Hitomi,
Bonfante Samara,
Garcia Valdir Gouveia
Publication year - 2008
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2008.080103
Subject(s) - medicine , saline , scaling and root planing , ligature , photodynamic therapy , periodontitis , diabetes mellitus , alloxan , periodontal disease , molar , dentistry , adjuvant , chronic periodontitis , chemistry , endocrinology , organic chemistry
Background: The aim of this study was to evaluate histologically and histometrically the influence of photodynamic therapy (PDT) as an adjuvant treatment on induced periodontitis in rats with diabetes. Methods: Two hundred forty rats were divided evenly into two groups: non‐diabetic (ND; n = 120) and alloxan diabetic (D; n = 120). Periodontal disease was induced in both groups at the first mandibular molar. After 7 days, the ligature was removed, and all animals underwent scaling and root planing (SRP) and were divided according to the following treatments: irrigation with saline solution (SRP); irrigation with a phenothiazinium dye (100 μg/ml) (TBO); laser irradiation (660 nm, 24 J) (LLLT); and PDT (TBO and laser irradiation). Ten animals in each experimental group and treatment subgroup were euthanized at 7, 15, and 30 days. The histometric values were analyzed statistically ( P <0.05). Results: In the ND group, the animals treated by PDT showed less bone loss (0.33 ± 0.05 mm 2 , 0.35 ± 0.06 mm 2 , and 0.27 ± 0.07 mm 2 at 7, 15, and 30 days, respectively; P <0.05) at all experimental periods than the SRP group (1.11 ± 0.11 mm 2 , 0.84 ± 0.12 mm 2 , and 0.97 ± 0.13 mm 2 at 7, 15, and 30 days, respectively), the TBO group (0.51 ± 0.12 mm 2 , 0.70 ± 0.13 mm 2 , and 0.64 ± 0.08 mm 2 at 7, 15, and 30 days, respectively), and the LLLT group (0.59 ± 0.03 mm 2 , 0.61 ± 0.04 mm 2 , and 0.60 ± 0.03 mm 2 at 7, 15, and 30 days, respectively). In the D group, the animals treated by PDT showed less bone loss (0.29 ± 0.03 mm 2 , 0.24 ± 0.02 mm 2 , and 0.27 ± 0.06 mm 2 at 7, 15, and 30 days, respectively; P <0.05) at all experimental periods than the SRP group (2.27 ± 0.47 mm 2 , 3.23 ± 0.34 mm 2 , and 2.82 ± 0.75 mm 2 at 7, 15, and 30 days, respectively), the TBO group (0.51 ± 0.15 mm 2 , 0.44 ± 0.07 mm 2 , and 0.57 ± 0.13 mm 2 at 7, 15, and 30 days, respectively), and the LLLT group (0.37 ± 0.05 mm 2 , 0.35 ± 0.09 mm 2 , and 0.39 ± 0.12 mm 2 at 7, 15, and 30 days, respectively). Conclusion: PDT was a beneficial adjuvant treatment for periodontal diseases induced by bacterial plaque and systemically modified by diabetes mellitus.