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Inflammatory Responses of Gingival Epithelial Cells Stimulated With Porphyromonas gingivalis Vesicles Are Inhibited by Hop‐Associated Polyphenols
Author(s) -
Kou Yurong,
Inaba Hiroaki,
Kato Takahiro,
Tagashira Motoyuki,
Honma Daiki,
Kanda Tomomasa,
Ohtake Yasuyuki,
Amano Atsuo
Publication year - 2008
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2008.070364
Subject(s) - porphyromonas gingivalis , chemistry , matrix metalloproteinase , prostaglandin e2 , periodontitis , microbiology and biotechnology , pharmacology , biology , biochemistry , medicine , endocrinology
Background: Periodontitis is induced by an imbalance between bacterial virulence and host defense ability. Porphyromonas gingivalis , a predominant periodontal pathogen, triggers a series of host inflammatory responses that aggravate the destruction of periodontium. Thus, anti‐inflammatory reagents are considered desirable for effective periodontal therapy. In the present study, we examined the inhibitory effects of hop bract polyphenol (HBP) on cellular inflammatory responses induced by P. gingivalis membrane vesicles. Methods: Immortalized human gingival epithelial cells were stimulated with P. gingivalis membrane vesicles, and the effects of HBP on mRNA expression of cyclooxygenase (COX)‐2, interleukin (IL)‐6 and −8, and matrix metalloproteinase (MMP)‐1 and −3 were examined using real‐time reverse transcription‐polymerase chain reaction. Results: HBP inhibited the mRNA expression of COX‐2, IL‐6 and −8, and MMP‐1 and −3 in a dose‐dependent manner, whereas epigallocatechin gallate (a control polyphenol) inhibited COX‐2 mRNA expression only. Following further fractionation of HBP to identify the effective components, 2‐[(2‐methylpropanoyl)‐phloroglucinol]1‐ O ‐β‐D‐glucopyranoside (MPPG) was identified as a significant anti‐inflammatory element that completely inhibited the inflammatory mRNA induction. Kaempferol 3‐ O ‐β‐glucopyranoside (astragalin) also was found to have anti‐inflammatory effects. Conclusions: HBP is suggested to be a potent inhibitor of cellular inflammatory responses induced by P. gingivalis vesicles. Further, MPPG and astragalin, identified here as effective components of HBP, also may be useful for the prevention and/or attenuation of periodontitis.