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Tannerella forsythia and Pseudomonas aeruginosa in Subgingival Bacterial Samples From Parous Women
Author(s) -
Persson G. Rutger,
Hitti Jane,
Paul Katie,
Hirschi Regula,
Weibel Marianne,
Rothen Marilynn,
Persson Rigmor E.
Publication year - 2008
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2008.070350
Subject(s) - tannerella forsythia , forsythia , pseudomonas aeruginosa , microbiology and biotechnology , medicine , biology , bacteria , pathology , honeysuckle , alternative medicine , traditional chinese medicine , genetics
Background: Information on the subgingival microbiota in parous women is limited. The present study assessed 74 bacterial species at periodontal sites. Methods: Subgingival bacterial plaque was collected from women ≥6 months after delivery. Bacteria were assessed by the checkerboard DNA‐DNA hybridization method. Gingivitis was defined as ≥20% of sites with bleeding on probing (BOP), and periodontitis was defined as radiographic evidence of bone loss and probing depths ≥5.0 mm. Results: A total of 197 women (mean age: 29.4 ± 6.8 years; range: 18 to 46 years) were included in the study. Gingivitis was identified in 82 of 138 subjects without evidence of periodontitis (59.4%). Periodontitis was found in 59 women (32%). Higher bacterial levels in subjects with gingivitis compared to those without evidence of gingivitis were observed for Actinomyces neuii , Bifidobacterium bifidum , Corynebacterium pseudogenitalis , Porphyromonas endodontalis , Prevotella bivia , and Pseudomonas aeruginosa ( P <0.001 for each). Higher bacterial levels in subjects with periodontitis compared to those without periodontitis (BOP not accounted for) were found for 32 of 79 species ( P <0.001) including Lactobacillus iners , Haemophilus influenzae , Porphyromonas gingivalis , Tannerella forsythia (previously T. forsythensis ), Prevotella bivia , P. aeruginosa , and Staphylococcus aureus . Binary univariate logistic regression analysis identified that P. aeruginosa ( P <0.001) and T. forsythia ( P <0.05) were independently predictive of periodontal status. The odds ratio of having P. aeruginosa at levels ≥1 × 10 5 in the sample and periodontitis was 3.1 (95% confidence interval: 1.6 to 5.9; P <0.001). Conclusion: In addition to P. gingivalis and T. forsythia , a diverse microbiota, including P. aeruginosa , P. endodontalis , P. bivia , and S. aureus , can be found in subgingival plaque samples from women of child‐bearing age with periodontitis.