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Nicotine and Lipopolysaccharide Affect Cytokine Expression From Gingival Fibroblasts
Author(s) -
Almasri Amjad,
Wisithphrom Kessiri,
Windsor L. Jack,
Olson Byron
Publication year - 2007
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2007.060296
Subject(s) - affect (linguistics) , nicotine , lipopolysaccharide , cytokine , porphyromonas gingivalis , medicine , periodontitis , dentistry , immunology , psychology , communication
Background: This in vitro study investigated the influence of nicotine, lipopolysaccharide (LPS), and a combination of both agents on cytokine expression from human gingival fibroblasts (HGFs). Methods: HGFs were exposed for 48 hours to 250 μg/ml nicotine, 1 μg/ml Porphyromonas gingivalis LPS, or both. The expression of multiple cytokines was detected in the HGFs conditioned media using cytokine protein arrays. Results: The untreated HGFs expressed several cytokines, which included relatively high levels of interleukin (IL)‐6, IL‐8, and monocyte chemoattractant protein‐1 (MCP‐1). They also expressed low levels of growth‐regulated oncogene (GRO), IL‐3, and IL‐10. Nicotine had the greatest effect on the expression of GRO‐α, IL‐7, IL‐10, and IL‐1‐ compared to the untreated control. P. gingivalis LPS had the greatest effect on the expression of GRO‐α; IL‐7; IL‐10; and RANTES (regulated on activation, normal T‐cell expressed, and presumably secreted) compared to the untreated control. The combination of both agents had the biggest impact on the expression of GRO‐α, IL‐7, IL‐10, IL‐15, RANTES, and interferon‐γ (IFN‐γ) compared to the untreated control. Conclusion: HGFs exposed to nicotine, P. gingivalis LPS, or a combination of both agents increased the expression of multiple cytokines.