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Hydrocortisone Affects the Expression of Matrix Metalloproteinases (MMP‐1, ‐2, ‐3, ‐7, and ‐11) and Tissue Inhibitor of Matrix Metalloproteinases (TIMP‐1) in Human Gingival Fibroblasts
Author(s) -
Cury Patricia R.,
Araújo Vera C.,
Canavez Flavio,
Furuse Cristiane,
Araújo Ney S.
Publication year - 2007
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2007.060225
Subject(s) - matrix metalloproteinase , matrix (chemical analysis) , chemistry , cancer research , microbiology and biotechnology , medicine , biology , biochemistry , chromatography
Background: There is a positive correlation between the course of periodontal disease and psychosocial stress status. Stress leads to activation of the hypothalamic‐pituitary‐adrenal axis, resulting in increased cortisol release. The present study evaluates the effect of two different hydrocortisone concentrations on mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinases (TIMPs) in cultured, human gingival fibroblasts. Methods: Gingival fibroblasts were stimulated with 10 −7 or 10 −9 M hydrocortisone for 24 hours; untreated cells served as controls. Alterations in the expression of MMP‐1, ‐2, ‐3, ‐7, ‐11 and TIMP‐1 and ‐2 were evaluated using real‐time polymerase chain reaction and Western blotting. β‐actin mRNA expression was used as a reference to normalize gene expression. Results: Although the higher hydrocortisone concentration upregulated MMP‐1, ‐2, ‐7, ‐11, and TIMP‐1 ( P <0.0‐) expression, the lower concentration induced downregulation or diminished upregulation. The lower hydrocortisone concentration induced a 23‐fold increase in MMP‐3 gene expression, whereas the higher concentration induced less upregulation; however, protein expression was regulated similarly by both hydrocortisone concentrations. The effect of hydrocortisone on TIMP‐2 expression was not significant ( P >0.0‐). Conclusions: Hydrocortisone produced a dose‐dependent regulation of MMP and TIMP expression. The higher hydrocortisone concentration significantly upregulated expression of MMP‐1, ‐2, ‐7, and ‐11 and TIMP‐1 in human gingival fibroblasts, which may constitute a mechanism underlying the increased periodontal breakdown associated with psychosocial stress status.