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Functional Polymorphisms in the Matrix Metalloproteinase‐9 Gene in Relation to Severity of Chronic Periodontitis
Author(s) -
Holla Lydie Izakovicova,
Fassmann Antonin,
Mužík Jan,
Vanek Jiri,
Vasku Anna
Publication year - 2006
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2006.050347
Subject(s) - odds ratio , allele , genotype , medicine , gastroenterology , chronic periodontitis , case control study , matrix metalloproteinase , periodontitis , confidence interval , polymorphism (computer science) , allele frequency , immunology , gene , genetics , biology
Background: Members of the matrix metalloproteinase (MMP) family are implicated in the chronic remodeling in periodontal diseases. Therefore, we performed a case‐control study to investigate a plausible association between susceptibility to chronic periodontitis (CP) and the polymorphisms in the MMP‐9 (gelatinase B) gene. Methods: Using polymerase chain reaction with subsequent restriction analyses, MMP‐9 −1562C/T and R+279Q variants were determined in 304 subjects (169 patients with CP, 76 with a mild to moderate form and 93 with severe generalized CP, and 135 age‐ and gender‐matched unrelated control subjects). Results: The distribution of the MMP‐9 −1562C/T and R+279Q genotypes and alleles did not significantly differ between cases and controls. However, the frequency of the T variant at position −1562 was marginally higher in patients with severe disease compared to those with mild to moderate forms ( P <0.05; P corr >0.05). In further analysis, the −1562T allele was associated with a severe form of CP in men (odds ratio: 3.87; 95% confidence interval: 1.40 to 10.65; P <0.01 and P corr <0.05 for allele; P <0.05 and P corr >0.05 for genotype) but not in women. Conclusion: These findings suggest that genetic polymorphisms of the MMP‐9 gene are not associated with the susceptibility to CP in the Czech population; however, the promoter variant may influence the severity of the disease in men.