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Autogenous Bone Graft With or Without a Calcium Sulfate Barrier in the Treatment of Class II Furcation Defects: A Histologic and Histometric Study in Dogs
Author(s) -
Deliberador Tatiana M.,
Nagata Maria J.H.,
Furlaneto Flávia A.C.,
Melo Luiz G.N.,
Okamoto Tetuo,
Sundefeld Maria L.M.M.,
Fucini Stephen E.
Publication year - 2006
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2006.050209
Subject(s) - furcation defect , medicine , dentistry , trichrome , connective tissue , debridement (dental) , h&e stain , molar , staining , pathology
Background: The purpose of this study was to histologically evaluate the healing of surgically created Class II furcation defects treated using an autogenous bone (AB) graft with or without a calcium sulfate (CS) barrier. Methods: The second, third, and fourth mandibular premolars (P2, P3, and P4) of six mongrel dogs were used in this study. Class II furcation defects (5 mm in height × 2 mm in depth) were surgically created and immediately treated. Teeth were randomly divided into three groups: group C (control), in which the defect was filled with blood clot; group AB, in which the defect was filled with AB graft; and group AB/CS, in which the defect was filled with AB graft and covered by a CS barrier. Flaps were repositioned to cover all defects. The animals were euthanized 90 days post‐surgery. Mesio‐distal serial sections were obtained and stained with either hematoxylin and eosin or Masson's trichrome. Histometric, using image‐analysis software, and histologic analyses were performed. Linear and area measurements of periodontal healing were evaluated and calculated as a percentage of the original defect. Percentage data were transformed into arccosine for statistical analysis (analysis of variance; P <0.05). Results: Periodontal regeneration in the three groups was similar. Regeneration of bone and connective tissue in the furcation defects was incomplete in most of the specimens. Statistically significant differences were not found in any of the evaluated parameters among the groups. Conclusion: Periodontal healing was similar using surgical debridement alone, AB graft, or AB graft with a CS barrier in the treatment of Class II furcation defects.