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Influence of Combinations of Bacteria on the Levels of Prostaglandin E 2 , Interleukin‐1β, and Granulocyte Elastase in Gingival Crevicular Fluid and on the Severity of Periodontal Disease
Author(s) -
AirilaMånsson Stella,
Söder Birgitta,
Kari Kirsti,
Meurman Jukka H.
Publication year - 2006
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2006.050208
Subject(s) - periodontitis , porphyromonas gingivalis , elastase , periodontal examination , medicine , immunology , chemistry , enzyme , biochemistry
Background: The aim of this study was to investigate the simultaneous presence of periodontal microbiota on inflammatory markers in gingival crevicular fluid from individuals with periodontal diseases. Methods: A total of 82 individuals with periodontal disease (mean age: 54.3 ± 3.0 years) and 31 periodontally healthy individuals (mean age: 53.2 ± 3.0 years) were randomly chosen and underwent clinical oral examinations in 2003 with the determination of the dental plaque index (PI), gingival index (GI), and periodontal probing depth (PD). The simultaneous presence of polymerase chain reaction (PCR)‐assessed periodontal bacteria, levels of prostaglandin E 2 (PGE 2 ), granulocyte elastase, interleukin 1‐beta (IL‐1β), and total protein concentration were determined from the pockets. Marginal bone height percent was measured on x‐rays. Analysis of variance and χ 2 tests were used to analyze the results. Results: In sites with Tannerella forsythensis , levels of PGE 2 (pg/site), granulocyte elastase (monoclonal antibodies (mAbs)/site), and total protein (mg/ml) were significantly higher than in sites without T. forsythensis ( P <0.05, P <0.01, and P <0.05, respectively). Those with periodontal disease with simultaneous presence of T. forsythensis and Porphyromonas gingivalis , or T. forsythensis and Prevotella nigrescens , showed significantly higher PI and GI, deeper PD, more loss of attachment, and more release of PGE 2 and granulocyte elastase than did periodontitis patients without these bacteria. Conclusion: The simultaneous presence of T. forsythensis and P. gingivalis , or T. forsythensis and P. nigrescens , seemed to promote the release of subgingival inflammatory mediators and seemed to be associated with more severe periodontal disease.