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Promotion of Functioning of Human Periodontal Ligament Cells and Human Endothelial Cells by Nerve Growth Factor
Author(s) -
Xu WanPeng,
Mizuno Noriyoshi,
Shiba Hideki,
Takeda Katsuhiro,
Hasegawa Naohiko,
Yoshimatsu Shinitiro,
Inui Takafumi,
Ozeki Yoshitaka,
Niitani Miyuki,
Kawaguchi Hiroyuki,
Tsuji Koichiro,
Kato Yukio,
Kurihara Hidemi
Publication year - 2006
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2006.050183
Subject(s) - paracrine signalling , nerve growth factor , periodontal fiber , endocrinology , medicine , autocrine signalling , microbiology and biotechnology , vascular endothelial growth factor , bone morphogenetic protein 2 , growth factor , chemistry , biology , receptor , in vitro , dentistry , biochemistry , vegf receptors
Background: We have previously shown that cultured human periodontal ligament (HPL) cells produce nerve growth factor (NGF) and express mRNA of tyrosine kinase receptor (trkA), a high‐affinity receptor of NGF. These findings suggest that NGF modulates the differentiation and proliferation of the periodontal ligament cells by paracrine and autocrine functions in vivo. Endothelial cells also express NGF and trkA. Therefore, NGF may regulate functions of periodontal ligament cells and endothelial cells during periodontal tissue regeneration. Methods: Effects of NGF on expressions of bone/cementum‐related proteins (osteocalcin [OC], bone sialoprotein [BSP], bone morphogenetic protein [BMP‐7], core binding factor alpha [Cbfa‐1], and type I collagen), calcification in HPL cells, and proliferation and mRNA expression of vascular endothelial growth factor (VEGF), an endothelial cell mitogen, in human microvascular endothelial cells (HMVECs) were examined. Results: NGF elevated mRNA levels of OC, BSP, BMP‐7, Cbfa‐1, and type I collagen and enhanced mineral deposition in cultures of HPL cells. Furthermore, NGF stimulated mRNA expressions of VEGF‐A and VEGF‐B and cell proliferation in HMVEC. Conclusion: These findings suggest that the functional regulation of periodontal ligament cells and endothelial cells by NGF might result in the acceleration of periodontal tissue regeneration in vivo.