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Collagenases in Gingival Crevicular Fluid in Type 1 Diabetes Mellitus
Author(s) -
SafkanSeppälä Bedia,
Sorsa Timo,
Tervahartiala Taina,
Beklen Arzu,
Konttinen Yrjö T.
Publication year - 2006
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2006.040322
Subject(s) - medicine , collagenase , dentistry , diabetes mellitus , endocrinology , chemistry , biochemistry , enzyme
Background: Studies have demonstrated that high levels of collagenase activity in gingival crevicular fluid (GCF) are associated with degradation of periodontal tissues in progressive periodontitis compared to periodontally healthy tissues. Because the activation of collagenases is an important issue in periodontitis, we have studied the activation of collagenase in gingival crevicular fluid samples of diabetic patients. Methods: Collagenase activity was studied in human gingival crevicular fluids. Twenty‐two poorly controlled diabetic patients (e.g., blood glucose: 11.0 ± 0.7 mmol/l; hemoglobin A 1c [HbA 1c ]: 9.6% ± 0.3%) and five well‐controlled diabetic patients were compared to six chronic periodontitis subjects and five healthy controls. Collagenase activity against type I collagen was measured using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis quantitated by laser densitometry. Results: The poorly controlled diabetic patients had more alveolar bone loss than the well‐controlled diabetic subjects and controls ( P <0.001; t test). The activity of collagenases in GCF in poorly controlled diabetic patients was similar to that seen in chronic periodontitis subjects ( P >0.05) but higher than in healthy controls ( P <0.01; t test), whereas there was no difference between the well‐controlled diabetic subjects and systemically healthy controls ( P >0.05; t test). Conclusion: Poorly controlled diabetes is strongly related to periodontal tissue destruction, and collagenases in GCF may mediate and reflect this effect.