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The Influence of Loss of Bone Mass on Induced Periodontal Disease: A Radiographic and Densitometric Study of Female Rats
Author(s) -
Orrico Silvana Regina Perez,
Gonçalves Daniela,
Galeazzi Solange Teresinha,
Giro Gabriela,
Takayama Lilian,
Pereira Rosa Maria Rodrigues
Publication year - 2005
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2005.76.9.1436
Subject(s) - bone mineral , medicine , femur , molar , mandible (arthropod mouthpart) , densitometry , dentistry , bone density , dual energy x ray absorptiometry , bone disease , periodontitis , ovariectomized rat , osteoporosis , mandibular second molar , estrogen , endocrinology , surgery , biology , botany , genus
Background: Changes in mineral density in the mandibular and femoral bones (BMD) after estrogen deficiency caused by ovariectomy (OVX) and the influence of these changes on induced periodontal disease were evaluated in female rats. Methods: Forty‐eight female Holtzman rats (90 days old) were randomly divided into five groups: 0: control (N = 9); 1: SHAM without induced periodontal disease (N = 11); 2: SHAM with induced disease (N = 10); 3: OVX without induced disease (N = 9); and 4: OVX with induced disease (N = 9). In groups 2 and 4, the first lower molars were tied with ligatures for 30 days 120 days after surgery. After 5 months the animals were sacri‐ ficed to measure global mineral density (BMD) and that of the sub‐regions of the mandible and femur by dual energy x‐ray absorptiometry (DXA). The extent of vertical bone loss was evaluated with digital radiography by measuring the distance from the bone crest to the cemento‐enamel junction at the mesial of the first lower molar. Results: Results of the femur (Kruskal‐Wallis test) showed a significant difference ( P <0.001) between the groups SHAM and OVX in bone density values for all regions. Comparison between the groups in relation to the BMD of the mandible, both in the sub‐regions and global revealed no differences ( P >0.05). The vertical bone loss measured for the groups with induced disease was similar ( P = 0.713). Conclusions: Differences between the groups were found in the bone mineral density BMD of the femur but not of the mandible. OVX had no influence on induced periodontal disease. J Periodontol 2005;76:1436‐1442 .