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Expression of Activated Transcription Factor Nuclear Factor‐κB in Periodontally Diseased Tissues
Author(s) -
Ambili R.,
Santhi W.S.,
Janam Prasanthila,
Nandakumar K.,
Pillai M. Radhakrishna
Publication year - 2005
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2005.76.7.1148
Subject(s) - transcription factor , microbiology and biotechnology , biology , genetics , gene
Background: Chronic periodontitis is an inflammatory disease found mainly in adults. Little is known about molecular level changes associated with host response in this condition. Nuclear factor‐κB (NF‐κB) is a transcription factor implicated in immune and inflammatory responses. NF‐κB activation has also been reported to be associated with many chronic inflammatory diseases. The purpose of this paper was to compare the nuclear and cytoplasmic expression of NF‐κB transcription factor (p50/p65) and cytoplasmic expression of IκB in periodontal tissues of periodontitis patients and controls. Methods: Twenty patients with chronic periodontitis and 20 healthy controls were included in the study. Gingival tissues taken during extraction were processed for immunohistochemical staining and evaluation. Results: Nuclear (activated) p50 was found in 90% of periodontal patient tissues compared to only 30% of healthy tissues. A more significant result was obtained with p65 (75% versus 5%). Intense cytoplasmic immunoreactivity was also observed in periodontitis tissues. IκB, the inhibitor of NF‐κB, was expressed only in 5% of periodontally diseased tissues. Conclusion: Activation of NF‐κB (p50/p65) is significant in periodontally diseased tissues, suggesting the potential of inhibitors of NF‐κB in managing periodontitis. J Periodontol 2005;76:1148‐1153 .