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Genetic Variations in the Human Gelatinase A (Matrix Metalloproteinase‐2) Promoter Are Not Associated With Susceptibility to, and Severity of, Chronic Periodontitis
Author(s) -
Holla Lydie Izakovicova,
Fassmann Antonin,
Vasku Anna,
Goldbergova Monika,
Beranek Michal,
Znojil Vladimír,
Vanek Jiri,
Vacha Jiri
Publication year - 2005
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2005.76.7.1056
Subject(s) - chronic periodontitis , haplotype , periodontitis , single nucleotide polymorphism , snp , pathogenesis , aggressive periodontitis , restriction fragment length polymorphism , immunology , medicine , gene , polymorphism (computer science) , biology , genetics , polymerase chain reaction , genotype
Background: Gelatinase A (matrix metalloproteinase‐2 [MMP‐2]) has been shown to play an important role in the pathogenesis of several disorders, including periodontal diseases. In this study, we test the hypothesis that variations in this gene influence the development and severity of chronic periodontitis. Methods: Four promoter polymorphisms (‐1575G/A, ‐1306C/T, ‐790T/G, and ‐735C/T) were analyzed by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) methods in 149 patients with mild to severe chronic periodontitis and 127 age‐matched controls in the Czech population. Results: No significant differences in distribution of the ‐1575G/A, ‐1306C/T, and ‐735C/T variants between periodontitis and control groups were detected in our study. However, a trend to decreased frequency of the ‐790 GG homozygotes was observed in patients with chronic periodontitis compared to healthy controls ( P = 0.036, P corr >0.05). Haplotype analysis of four single nucleotide polymorphisms (SNP) in the MMP‐2 gene showed no significant association of any haplotype with chronic periodontitis. Conclusion: Our findings suggest that polymorphisms in the MMP‐2 gene promoter do not contribute significantly to the interindividual periodontitis susceptibility and/or severity in European Caucasians, and they are not regulatory variants in this disease. J Periodontol 2005;76:1056‐1060 .