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Biomimetic Approach on Human Periodontal Ligament Cells Using Synthetic Oligopeptides
Author(s) -
Kim TaeII,
Jang JunHyeog,
Lee YongMoo,
Rhyu InChul,
Chung ChongPyoung,
Han SooBoo,
Choi SangMook,
Ku Young
Publication year - 2004
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2004.75.7.925
Subject(s) - periodontal fiber , fibronectin , chemistry , cementum , peptide , microbiology and biotechnology , cell adhesion , cell culture , cell , biochemistry , biology , dentin , pathology , dentistry , medicine , genetics
Background: Periodontal ligament (PDL) cells, connecting root cementum with alveolar bone, are important for periodontal wound healing. In order to obtain a predictable periodontal regeneration, selective adhesion and proliferation of PDL cells are essential. The purpose of this study was to investigate the effects of synthetic peptides mimicking cell‐binding domain of fibronectin (FN) on human PDL cells. Methods: Two types of oligopeptides, Gly 3 ‐Pro‐His‐Ser‐Arg‐ Asn‐Gly 6 ‐Arg‐Gly‐Asp‐Gly (G 3 PHSRNG 6 RGDG) and Gly 3 ‐His‐ Pro‐Asn‐Arg‐Ser‐Gly 6 ‐Arg‐Gly‐Asp‐Gly (G 3 HPNRSG 6 RGDG), were constructed using a solid‐phase peptide synthesizer. Fibronectin type III ninth to tenth domain (FN III 9‐10) and Arg‐Gly‐Asp‐Ser (RGDS) were prepared for comparison with the effects of synthetic peptides. These peptides were coated onto 96‐well cell culture plates with 0.001 ∼ 100 μM concentrations. Cultured human PDL cells were then applied to the peptide‐coated wells at a density of 1 × 10 4 /well. After 1 hour incubation at 37°C, adhered cells were fixed, stained, and examined by phase contrast microscopy for cell spreading assay. Attached PDL cells were solubilized with 2% sodium dodecyl sulfate (SDS) for the cell attachment assay by measuring absorbance at 595 nm in microplate reader. Western blot analysis was performed to determine extracellular signal‐regulated kinase (ERK1/2) activity. Results: Cell attachment and spreading assays revealed that G 3 PHSRNG 6 RGDG (≥10 μM) possesses similar adhesive behavior to FN III 9‐10. G 3 PHSRNG 6 RGDG showed a comparable ERK1/2 activity when compared to FN III 9‐10. Conclusions: G 3 PHSRNG 6 RGDG enhanced an attachment and spreading of human PDL cells thereby increasing ERK1/2 activity. Taken together, it is anticipated that this peptide might be a potential tool for arranging a biologically attractive environment for PDL cells, which would enhance periodontal regeneration efficacy. J Periodontol 2004;75:925‐932 .