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The Influence of Enamel Matrix Derivative Associated With Insulin‐Like Growth Factor‐I on Periodontal Ligament Fibroblasts
Author(s) -
Palioto Daniela B.,
Coletta Ricardo D.,
Graner Edgard,
Joly Julio Cesar,
Lima Antonio Fernando Martorelli
Publication year - 2004
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2004.75.4.498
Subject(s) - enamel matrix derivative , periodontal fiber , enamel paint , growth factor , chemistry , dentistry , medicine , microbiology and biotechnology , biology , regeneration (biology) , receptor
Background: Enamel matrix derivative (EMD) has recently been shown to promote periodontal regeneration in vivo. Insulin‐like growth factor‐I (IGF‐I) is a potent modulator of periodontal regeneration stimulating cell proliferation, differentiation, synthesis of type I collagen, and non‐collagenous proteins. However, the biochemical effects of these factors on periodontal ligament (PDL) fibroblasts are not completely understood. The objective of the present study was to evaluate the effect of EMD, IGF‐I, and the combination of these two factors on the proliferation, adhesion, migration, and expression of type I collagen in PDL fibroblasts. Methods: The proliferation rate was measured by automated cell counting and immunohistochemical expression of proliferating cell nuclear antigen (PCNA). The cell adhesion was analyzed by a colorimetric assay and cell migration was measured in Boyden chambers. Type I collagen expression and production was determined by semi‐quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) and enzyme‐linked immunosorbent assay (ELISA), respectively. Results: The results indicated that the proliferation of PDL fibroblasts was significantly stimulated by EMD and EMD plus IGF‐I in a dose‐ and time‐dependent manner. EMD, IGF‐I, and the combination of both factors had no effects on cellular migration and adhesion or expression and production of type I collagen. Conclusion: Our results showed that EMD, IGF‐I, and the combination of both factors stimulated PDL fibroblast proliferation, whereas these factors did not affect adhesion, migration, or expression of type I collagen of these cells. J Periodontol 2004;75:498‐504 .