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Analysis of the Interleukin‐6 Gene Promoter Polymorphisms in Czech Patients with Chronic Periodontitis
Author(s) -
Holla Lydie Izakovicova,
Fassmann Antonin,
Stejskalová Andrea,
Znojil Vladimír,
Vaněk Jiri,
Vacha Jiri
Publication year - 2004
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2004.75.1.30
Subject(s) - chronic periodontitis , haplotype , periodontitis , genotype , allele , heterozygote advantage , odds ratio , proinflammatory cytokine , immunology , biology , interleukin , tooth loss , medicine , genetics , gene , inflammation , cytokine , dentistry , oral health
Background: Chronic periodontitis is an inflammatory disease, which is a major cause of tooth loss. The proinflammatory cytokines interleukin‐1 (IL‐1) and interleukin‐6 (IL‐6) are key regulators of the host response to microbial infection and major modulators of extracellular matrix catabolism and bone resorption. The purpose of this study was to investigate the associations of chronic periodontitis with IL‐6 gene polymorphisms (at positions –597 [G/A], –572 [G/C], and –174 [G/C]). Methods: We analyzed allele, genotype, and haplotype distributions of the IL‐6 promoter variants in a case‐control study involving 148 patients with chronic periodontitis and 107 unrelated controls. Results: Our results showed significant differences in the distributions of alleles and genotypes of the IL‐6 (–572 G/C) polymorphism between patients and the control population (X 2 = 10.393, P = 0.001, P corr <0.01). The difference was due to the underrepresentation of the –572 G/C heterozygotes in patients (6.1%) compared to controls (19.6%). Although no variant “CC” homozygotes were detected in our cases and controls, heterozygosity protected against chronic periodontitis, representing a 73% reduction of risk (odds ratio [OR] = 0.27, 95% con‐ fidence interval: 0.12‐0.61) compared to wild‐type homozygotes. However, there were no significant differences in genotype or allele frequencies between both groups for IL‐6 –597 G/A and –174 G/C polymorphisms. Conclusion: This study is the first, to our knowledge, suggesting that the –572 G/C polymorphism of the IL‐6 gene may be one of the protective factors associated with lower susceptibility to chronic periodontitis. J Periodontol 2004;75:30‐36 .