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Distribution of Interleukin‐1β +3954 and IL‐1α –889 Genetic Variations in a Thai Population Group
Author(s) -
Anusaksathien Orasa,
Sukboon Angkana,
Sitthiphong Piyawan,
Teanpaisan Rawee
Publication year - 2003
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2003.74.12.1796
Subject(s) - group (periodic table) , distribution (mathematics) , population , medicine , chemistry , mathematics , environmental health , mathematical analysis , organic chemistry
Background: The severe form of chronic periodontitis (CP) has been reported to be strongly associated with the presence of allele 2 of composite IL‐1β +3954 and IL‐1α –889 genetic polymorphisms (genotype positive). However, other studies have reported conflicting findings, not only on the association between the composite IL‐1 gene polymorphisms and CP, but also the link between IL‐1 gene polymorphisms and aggressive periodontitis (AgP). These might have resulted from differences in ethnic background and disease entities. The aim of this study was to determine the distribution of IL‐1β +3954 and IL‐1α –889 genetic polymorphisms in a group of Thai subjects based on their periodontal status, including CP, AgP, and healthy groups. Methods: A total of 123 Thai subjects were clinically and radiographically assessed for their periodontal status. Blood samples were collected by fingerstick and adsorbed onto filter paper. The IL‐1β +3954 and IL‐1α –889 genotypes were performed by polymerase chain reaction, digested with restriction enzymes, and separated by gel electrophoresis. Results: The distribution of allele 1 homozygous genotype was 97.6% and 84.6% for IL‐1β +3954 and IL‐1α –889 , respectively. No allele 2 homozygous genotype was detected in either of these two gene loci. Only 1.6% (2 out of 123) of the subjects were genotype positive, which was too low to determine the association between the composite genotype of IL‐1β +3954 and IL‐1α –889 and severe forms of periodontal disease. Conclusion: Genetic polymorphism of IL‐1 genes in these two loci may not be useful in predicting the severity of periodontal disease in the Thai ethnic group. J Periodontol 2003;74:1796‐1802 .