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Gene Expression of Extracellular Matrix Proteoglycans in Human Cyclosporin‐Induced Gingival Overgrowth
Author(s) -
Gnoatto Nelson,
Lotufo Roberto F.M.,
Toffoletto Odaly,
Marquezini Mônica V.
Publication year - 2003
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2003.74.12.1747
Subject(s) - extracellular matrix , gene expression , gene , microbiology and biotechnology , extracellular , matrix metalloproteinase , matrix (chemical analysis) , chemistry , biology , biochemistry , chromatography
Background: Gingival overgrowth is one of the side effects associated with the systemic use of cyclosporin A (CsA). In vitro studies on the extracellular matrix of gingival tissues have demonstrated an altered composition, particularly an accumulation of proteoglycans and collagen. We investigated the gene expression of extracellular matrix proteoglycans in CsA‐induced gingival tissue alterations. Methods: mRNA expression of the proteoglycans perlecan, decorin, biglycan, and versican was analyzed by reverse transcription polymerase chain reaction (RT‐PCR) in gingival samples obtained from 12 individuals, six with CsA‐induced gingival overgrowth (CsA group) and six with a normal gingiva (control group). The RT‐PCR products were subjected to 1% agarose gel electrophoresis containing ethidium bromide and analyzed qualitatively and semiquantitatively by densitometry. Density values were normalized by determining the expression of the housekeeping gene ß‐actin in the same sample. Groups were compared by the Student's t test. Results: Perlecan expression showed a marked increase (54%) in the CsA group compared to the control group ( P <0.01), while no significant differences were observed for the other proteoglycans. Conclusion: CsA‐induced gingival overgrowth seems to be associated with increased expression of perlecan, a typical basement membrane proteoglycan, but not decorin, biglycan, or versican. J Periodontol 2003;74:1747‐1753 .