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Effects of Serum on Hepatocyte Growth Factor Secretion and Activation by Periodontal Ligament and Gingival Fibroblasts
Author(s) -
Ohshima Mitsuhiro,
Yokosuka Ryoji,
Yamazaki Yoshitaka,
Tokunaga Tsuyoshi,
Maeno Masao,
Otsuka Kichibee
Publication year - 2002
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2002.73.5.473
Subject(s) - hepatocyte growth factor , paracrine signalling , fetal bovine serum , chemotaxis , secretion , periodontal fiber , chemistry , growth factor , endocrinology , cell culture , medicine , basic fibroblast growth factor , fibroblast , in vitro , biology , biochemistry , dentistry , receptor , genetics
Background: Hepatocyte growth factor (HGF)/scatter factor is a paracrine growth factor secreted by mesenchymal cells, which exerts an effect on a variety of epithelial cell types. Our recent study revealed that periodontal ligament fibroblasts (PLF) and gingival fibroblasts (GF) cultured in the presence of serum which contains various stimulants produced HGF or HGF‐like factor, a predominant chemoattractant for gingival epithelial cells, and suggested that it could be involved in epithelial downgrowth in periodontitis. Methods: To clarify whether serum in medium stimulates PLF and GF to synthesize or activate HGF, the effect of fetal bovine serum (FBS) on HGF production was determined by enzymelinked immunoabsorbent assay (ELISA), and its chemotactic activity for gingival epithelial cells was examined by modified Boyden chamber assay. Results: One to 10% FBS in the culture medium stimulated HGF secretion in a dose‐dependent manner and the chemotactic activity was decreased by treatment with anti‐hHGF neutralizing antibody. Furthermore, fibroblast‐conditioned medium incubated with FBS and aprotinin reduced its chemotactic activity. Interestingly, serum‐free culture of PLF and GF produced potent chemoattractants for gingival epithelial cells other than HGF. Conclusions: These results show that FBS stimulates both HGF secretion and activation by PLF and GF. J Periodontol 2002;73:473‐478.