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The Effect of Duration and Force of Mechanical Toothbrushing Stimulation on Proliferative Activity of the Junctional Epithelium
Author(s) -
Tomofuji Takaaki,
Morita Manabu,
Horiuchi Masazumi,
Sakamoto Tomonori,
Ekuni Daisuke,
Yamamoto Tatsuo,
Watanabe Tatsuo
Publication year - 2002
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2002.73.10.1149
Subject(s) - junctional epithelium , proliferating cell nuclear antigen , epithelium , basal (medicine) , quadrant (abdomen) , stimulation , chemistry , pathology , andrology , medicine , immunohistochemistry , dentistry , insulin
Background: Gingival bleeding is frequently accompanied by an ulcerated epithelium, with repair depending on the proliferative activity of the epithelial cells. The present study examined the proliferative activity of the junctional epithelium (JE) under several different methods of toothbrushing stimulation. Methods: Twelve dogs were used in this 3‐week experiment. Prior to the experiment, all teeth underwent daily removal of plaque and calculus using a scaler. Teeth were then divided into quadrants: 9 teeth in 3 quadrants (test, 3 per quadrant) were mechanically stimulated by toothbrushing for various time intervals (10, 20, or 40 seconds) and at various forces (100, 200, or 250 gf). The 3 teeth in the fourth quadrant served as controls. The proliferative activity of the basal cells of the junctional epithelium was evaluated for expression of proliferating cell nuclear antigen (PCNA). The numbers of PCNA‐positive basal cells and total basal cells were counted, and the width of the junctional epithelium was measured. Results: Toothbrushing force significantly affected the PCNApositive basal cell ratio ( P <0.05). The 200 g toothbrushing force produced the highest PCNA‐positive basal cell ratio, which was significantly higher than that using the 100 g force ( P <0.05). Conclusion: In junctional epithelium stimulated with a toothbrush, the PCNA‐positive basal cell ratio is more sensitive to toothbrushing force than to duration. J Periodontol 2002;73:1149‐1152.