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Hyaluronan Supports Recombinant Human Bone Morphogenetic Protein‐2 Induced Bone Reconstruction of Advanced Alveolar Ridge Defects in Dogs. A Pilot Study
Author(s) -
Hunt Dennis R.,
Jovanovic Sascha A.,
Wikesjö Ulf M.E.,
Wozney John M.,
Bernard George W.
Publication year - 2001
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2001.72.5.651
Subject(s) - alveolar ridge , radiodensity , bone morphogenetic protein 2 , bone morphogenetic protein , dentistry , dental alveolus , medicine , implant , bone formation , surgery , radiography , chemistry , in vitro , biochemistry , gene
Background: Prosthetic‐driven implant dentistry requires predictable procedures for alveolar ridge augmentation. The objective of this pilot study was to evaluate bone regeneration in mandibular, full‐thickness, alveolar ridge, saddle‐type defects following surgical implantation of recombinant human bone morphogenetic protein‐2 (rhBMP‐2) in a novel hyaluronan (HY) sponge carrier. This sponge was fabricated from auto‐crosslinked HY. Methods: Alveolar ridge defects (∼15 × 10 × 10 mm), 2 per jaw quadrant, were surgically prepared in each of 3 young adult American fox hounds. Four defects were immediately implanted with rhBMP‐2/HY. Three defects were implanted with rhBMP‐2 in an absorbable collagen sponge (ACS) carrier (positive control). The rhBMP‐2 solution (1.5 ml at 0.2 mg/ml) was soak‐loaded onto the HY and ACS sponges. Three defects were implanted with HY sponges soak‐loaded with buffer without rhBMP‐2 (negative control), while 2 defects served as surgical controls. The animals were euthanized at 12 weeks postsurgery for histometric analysis. Results: Clinically, alveolar ridge defects receiving rhBMP‐2/ACS exhibited a slight supracrestal expansion, while defects receiving rhBMP‐2/HY were filled to contour. In contrast, the HY and surgical controls exhibited ridge collapse. rhBMP‐2/HY‐treated defects exhibited a dense bone quality without radiolucent regions observed in defects treated with rhBMP‐2/ACS. The histometric analysis showed 100% bone fill for the rhBMP‐2/ACS defects and 94%, 58%, and 65% bone fill for the rhBMP‐2/HY, HY, and surgical control defects, respectively. Conclusions: The conclusions are based on data from 2 of 3 animals in the study. In one animal, no response to rhBMP‐2 was observed with either carrier, and the animal may have been a non‐responder of unknown nature. With this limitation, the observations herein suggest that: 1) HY supports significant bone induction by rhBMP‐2; 2) the rhBMP‐2–induced bone assumes qualities of the immediate resident bone; 3) HY alone exhibits no apparent osteoconductive potential; and 4) HY appears to resorb within a 12‐week healing interval in the absence or presence of rhBMP‐2. Thus, HY appears to be a suitable candidate carrier for rhBMP‐2. J Periodontol 2001;72:651‐658.