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Some Effects of Non‐Surgical Therapy on Gingival Inflammatory Cell Subsets in Patients With Early‐Onset Periodontitis Associated With Actinobacillus actinomycetemcomitans
Author(s) -
Kleinfelder Jörg W.,
Sculean Anton,
Lange Dieter E.
Publication year - 2001
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2001.72.12.1713
Subject(s) - actinobacillus , periodontitis , medicine , alkaline phosphatase , statistical significance , polyclonal antibodies , antibody , scaling and root planing , acid phosphatase , cd20 , immunology , pathology , dentistry , biology , chronic periodontitis , enzyme , biochemistry
Background: Limited information is available as to whether local cellular immunity in early‐onset periodontitis (EOP) subjects harboring Actinobacillus actinomycetemcomitans ( Aa ) differs from that in patients without Aa . In addition, the effect of scaling and root planing on various lymphocyte subsets is described rather sparsely. Methods: In 10 subjects with early‐onset periodontitis harboring Aa (EOP‐ Aa ) and in 10 subjects without Aa (EOP‐non Aa ), clinical measurements were recorded and gingival biopsies were performed before and after scaling and root planing. The specimens were cut into serial sections; using the alkaline phosphatase‐antialkaline phosphatase technique, monoclonal antibodies to CD20 (B cells), CD30 (plasma cells), and CD45RO (T‐memory cells) were applied as well as polyclonal antibodies to alpha, gamma, and mu chains (Ig A, G, and M). Cells were counted from an area of 0.25 mm 2 in areas showing the largest infiltration. Results: Before therapy, mean counts of all cell phenotypes were found to be markedly enhanced in the EOP‐ Aa group compared to EOP‐non Aa subjects. Following scaling and root planing, the numbers of all phenotypes decreased in both groups. However, comparing the data before and after therapy in the EOP‐ Aa group, the P value was <0.05 only for CD30‐positive cells. In the EOP‐non Aa group, the differences before and after therapy reached statistical significance ( P <0.05) for all cell counts, except for IgMpositive cells. Furthermore, reduction of probing depth and gain of clinical attachment reached the 0.05 level of statistical significance only in EOP‐non Aa subjects. Conclusions: In EOP subjects harboring Aa , inflammatory cell subsets were detected in 2‐ to 3‐fold higher numbers compared to patients without Aa . Scaling and root planing resulted in a decrease of all cell phenotypes studied in individuals without Aa , whereas in subjects with Aa , the only significant decrease that was seen occurred in plasma cells. J Periodontol 2001;72:1713‐1719.