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Effect of Diode Laser Irradiation on the Attachment Rate of Periodontal Ligament Cells: An In Vitro Study
Author(s) -
Kreisler Matthias,
Meyer Christine,
Stender Elmar,
Daubländer Monika,
WillershausenZönnchen Britta,
d'Hoedt Bernd
Publication year - 2001
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2001.72.10.1312
Subject(s) - periodontal fiber , molar , laser , dentistry , materials science , in vitro , irradiation , root canal , chemistry , biomedical engineering , medicine , optics , biochemistry , physics , nuclear physics
Background: The present study is part of a basic research program investigating the cellular effects of an 810 nm GaAlAsdiode laser on human periodontal tissues. The aim of the investigation was to evaluate the effects of laser treatment of root surface specimens on the attachment of periodontal ligament (PDL) cells in vitro. Methods: Root specimens were prepared from periodontally diseased teeth. PDL cells were obtained from human third molar ligaments. Cells were cultured under simple, standardized, and reproducible experimental conditions. One hundred fifty root specimens were scaled and root planed with curets followed by air‐powder abrasive treatment; 75 were then lased and 75 served as controls. The irradiation time was 20 seconds at a power output of 1 W. The root segments were placed into culture dishes, covered with a solution of PDL cells, and incubated for 72 hours. The specimens were then washed with phosphate buffer to remove cells not attached to the surface, and the adherent cells were stained with methylene blue. Cells were counted using a reflected light microscope and the cell density per mm 2 was calculated. Results: The analysis of 150 specimens revealed no signifi‐cant differences between the groups ( P = 0.347, Wilcoxon test). The cell numbers, however, were slightly higher on laser specimens. The mean was 66 cells/mm 2 in the laser group and 63.7 cells/mm 2 in the control group. Conclusions: The application of the diode laser at the parameters used did not have a substantially positive effect on the new attachment of PDL cells on the tooth specimens. It remains to be investigated whether the difference detected is really clinically relevant. J Periodontol 2001;72:1312‐1317.