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Effects of Whole Cell Sonicates of Treponema lecithinolyticum on Osteoclast Differentiation
Author(s) -
Choi BongKyu,
Ohk SeungHo,
Lee Hyun Jung,
Kang Jung Hwa,
Jeong Gook Jin,
Yoo YunJung
Publication year - 2001
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2000.72.9.1172
Subject(s) - osteoclast , bone resorption , acid phosphatase , chemistry , tartrate resistant acid phosphatase , haematopoiesis , multinucleate , cellular differentiation , resorption , microbiology and biotechnology , bone marrow , prostaglandin e2 , biochemistry , immunology , biology , endocrinology , stem cell , in vitro , enzyme , gene
Background: Alveolar bone destruction is a characteristic feature of periodontal diseases and multinucleated osteoclast cells derived from hemopoietic cells are responsible for bone resorption. Treponema lecithinolyticum is a novel oral spirochete isolated from the periodontal lesions. Methods: The effect of whole cell sonicates on the osteoclast differentiation was examined in a co‐culture system of hemopoietic mouse bone marrow cells and calvaria derived‐osteoblastic cells to clarify the role of T. lecithinolyticum in the alveolar bone destruction associated with periodontal diseases. The differentiated osteoclasts were confirmed by tartrate‐resistant acid phosphatase (TRAP) staining. Results: Sonicates of this bacterium stimulated the osteoclast formation in the co‐culture system in a dose‐dependent manner. The sonicates‐induced osteoclast formation was partially inhibited by the heat treatment of sonicates. Indomethacin, which is a prostaglandin inhibitor, decreased the osteoclast formation induced by the bacterial sonicates. Conclusions: These findings suggest that T. lecithinolyticum induces osteoclast differentiation by a prostaglandin E2‐dependent mechanism and that heat‐labile components may be involved in this process. J Periodontol 2001;72:1172‐1177.