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The Mouthwash: A Non‐Invasive Sampling Method to Study Cytokine Gene Polymorphisms
Author(s) -
Laine Marja L.,
Farré Maria A.,
Crusius J. Bart A.,
Winkelhoff ArieJan,
Peña A. Salvador
Publication year - 2000
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2000.71.8.1315
Subject(s) - variable number tandem repeat , gene polymorphism , polymerase chain reaction , genomic dna , gene , interleukin 1 receptor antagonist , biology , polymorphism (computer science) , genotype , genetics , receptor antagonist , receptor , antagonist
Background: We describe a simple, non‐invasive mouthwash sampling method for rapid DNA isolation to detect cytokine gene polymorphisms. In the present paper, interleukin‐1β ( IL‐1B ) and interleukin‐1 receptor antagonist ( IL‐1RN ) gene polymorphisms were studied. Methods: Two mouthwash samples and blood samples were collected from 11 healthy individuals. The second mouthwash sample was stored for 7 days at room temperature. Polymerase chain reaction amplification was used to identify a bi‐allelic polymorphism at position +3953 in the IL‐1B gene and a variable number of tandem repeats (VNTR) polymorphism in the IL‐1RN gene. Results: Our results show that the typing of these cytokine gene polymorphisms using DNA isolated from mouthwash samples did not differ from those obtained by a phenol/chloroform isolation method from EDTA anti‐coagulated blood. Moreover, reliable results from mouthwash samples were obtained after storage for at least 7 days at room temperature. Conclusions: Mouthwash can be the method of choice to study gene polymorphisms in periodontitis and other chronic inflammatory diseases. J Periodontol 2000;71:1315‐1318.