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Inverse Dose‐ and Time‐Dependent Effect of Basic Fibroblast Growth Factor on the Gene Expression of Collagen Type I and Matrix Metalloproteinase‐1 by Periodontal Ligament Cells in Culture
Author(s) -
Palmon A.,
Roos H.,
Edel J.,
Zax B.,
Savion N.,
Grosskop A.,
Pitaru S.
Publication year - 2000
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2000.71.6.974
Subject(s) - periodontal fiber , matrix metalloproteinase , fibroblast , matrix (chemical analysis) , basic fibroblast growth factor , type i collagen , gene expression , gene , cell culture , chemistry , microbiology and biotechnology , growth factor , biology , dentistry , medicine , endocrinology , biochemistry , genetics , receptor , chromatography
Background: Growth factors are known to play a major role in the regeneration of the periodontium. Basic fibroblast growth factor (bFGF) is a polypeptide growth factor considered to have a role in chemotaxis and mitogenesis of periodontal ligament cells (PLC). The aim of this study was to assess the dose‐dependent effect of bFGF administration on the levels of gene expression of collagen type I (a1) (col I), collagen type III (col III), and collagenase‐1 (MMP‐1) in PLC. Methods: PLC were cultured in different concentrations of bFGF (0.1 to 10 ng of bFGF) for 14 and 21 days. At each time point, the gene expression of the examined molecules was assessed semi‐quantitatively by reverse transcription‐polymerase chain reaction (RT‐PCR) assay. Results: The results indicated that bFGF exhibits an inverse time‐ and dose‐dependent effect on the gene expression of col I and MMP‐1: it simultaneously downregulates the gene expression of col I and upregulates the gene expression of MMP‐1. On the other hand, bFGF had no dose‐dependent effect on col III gene expression. The effect of bFGF on the expression of the three genes was modulated by the time of incubation with bFGF. Conclusions: These results suggest that bFGF is one of the important regulators involved in the active remodeling of col I in the periodontal ligament and possibly in other connective tissues. J Periodontol 2000;71:974‐980.