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Interleukin‐8 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis
Author(s) -
Jin Lijian,
Söder Birgitta,
Corbet Esmonde F.
Publication year - 2000
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.2000.71.6.929
Subject(s) - periodontitis , elastase , treponema denticola , granulocyte , medicine , chronic periodontitis , gastroenterology , chemistry , immunology , porphyromonas gingivalis , enzyme , biochemistry
Background: This study aimed to determine the relationships among interleukin (IL)‐8 and granulocyte elastase levels in gingival crevicular fluid (GCF) and the concomitant presence of periodontopathogens in untreated adult periodontitis. Methods: GCF and subgingival plaque samples were collected from 16 patients with untreated adult periodontitis and 10 healthy control subjects. IL‐8 levels were determined by enzymelinked immunosorbent assay (ELISA). Granulocyte elastase was analyzed with a neutrophilic granulocyte‐specific, low molecular weight and chromogenic substrate, L‐pyroglutamyl‐Lprolyl‐L‐valine‐p‐nitroanilide, and the maximal rate of elastase activity (MR‐EA) was calculated. Five DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.). Results: Lower IL‐8 concentrations and higher granulocyte elastase activities were found in patients than in healthy controls as well as in diseased conditions co‐infected with B.f., P.g., P.i., and T.d. as compared to healthy conditions without the target species ( P <0.05). IL‐8 concentrations were positively correlated with MR‐EA levels in the periodontitis conditions coinfected with B.f., P.g., P.i., and T.d. ( P <0.05). A wide range of IL‐8 concentrations was found among 15 patients when the periodontitis condition was characterized by co‐infection with B.f., P.g., P.i., and T.d. MR‐EA levels in the high IL‐8 group of subjects were significantly higher than those in the low IL‐8 group of subjects ( P <0.01). Conclusions: The present study shows that the local hostbacteria interactions in untreated periodontitis are diverse in terms of the intensity of inflammatory responses measured by IL‐8–related granulocyte elastase activity in GCF. This might reflect different phases of the inflammatory response due to shifts in host‐bacteria interactions and therefore be indicative of a range of periodontal disease activity levels. J Periodontol 2000;71:929‐939.