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Periodontal Pathogens Stimulate CC‐Chemokine Production by Mononuclear and Bone‐Derived Cells
Author(s) -
Jiang Yanling,
Graves Dana T.
Publication year - 1999
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1999.70.12.1472
Subject(s) - chemokine , porphyromonas gingivalis , peripheral blood mononuclear cell , chemotaxis , monocyte , actinobacillus , ccl7 , macrophage inflammatory protein , microbiology and biotechnology , immunology , chemistry , ccl2 , inflammation , biology , periodontitis , in vitro , medicine , biochemistry , receptor
Background: Chemokines are chemotactic cytokines that stimulate recruitment of leukocytes. Monocyte chemoattractant protein‐1, macrophage inflammatory protein‐1α, and RANTES (regulated on activation, normal T cell expressed, and secreted) are 3 well‐characterized CC‐chemokines that regulate mononuclear cell recruitment. The recruitment of inflammatory cells is of particular importance in the oral cavity because of the likelihood that cells will be challenged with bacteria either during acute infection or following surgical procedures. Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans are putative periodontal pathogens that may be harbored in subgingival and supragingival plaque. The capacity of the host to respond to these bacteria by the elaboration of chemoattractants may represent an important defense mechanism. Methods: In the present study, we examined CCchemokine production by human mononuclear cells and bone‐derived cells in response to P. gingivalis, A. actinomycetemcomitans and lipopolysaccharides (LPS) stimulation in vitro. The chemokines produced were measured by ELISA. Results: The results demonstrate that P. gingivalis and A. actinomycetemcomitans induce high levels of MIP‐1α in mononuclear cells. P. gingivalis and A. actinomycetemcomitans stimulated high levels of MCP‐1 in bone‐derived cells and induced moderate levels of RANTES production in both mononuclear and osteoblastic cells. In mononuclear cells, LPS induced high levels of MIP‐1α and RANTES and moderate levels of MCP‐1; in osteoblasts LPS only induced MCP‐1. Conclusions: The capacity of bacteria to induce a given chemokine depends upon the cell type stimulated. That different cell types would exhibit differences in the CC‐chemokines produced under the same stimulus provides a mechanism to explain tissue‐specific recruitment of leukocytes. J Periodontol 1999;70:1472‐1478.