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Human Periodontal Ligament Fibroblast Behavior on Chemically Conditioned Dentine: An In Vitro Study
Author(s) -
Rompen Eric H.,
Goffinet Gerhard H.,
Nusgens Betty
Publication year - 1999
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1999.70.10.1144
Subject(s) - periodontal fiber , citric acid , cementum , conditioning , minocycline , chemistry , dentinal tubule , periodontitis , in vitro , connective tissue , dentistry , microbiology and biotechnology , biochemistry , dentin , biology , medicine , pathology , statistics , mathematics , antibiotics
Background: Chemical root conditioning is widely used in an attempt to improve the outcome of regenerative periodontal surgery, but its effect on connective tissue cell proliferation and biosynthetic activity has been poorly studied. The goal of the present study was to test in vitro the consequences of conditioning human dentine by citric acid or minocycline on the behavior of attached human periodontal ligament (HPDL) cells in terms of proliferation, protein synthesis and morphological appearance. Methods: HPDL cells were seeded on powdered human dentine, either untreated or conditioned for 3 minutes with 3% citric acid or 2.5% minocycline HCl. Scanning (SEM) and transmission (TEM) electron microscopic observations were performed, and 3 H‐thymidine and 3 H‐proline incorporation tests were used to evaluate the proliferative and the biosynthetic activities. Results: Cell spreading was already evident and the penetration of cytoplasmic processes into dentinal tubules were frequently observed on all dentine types after 2 hours of attachment. After 24 hours of incubation, citric acid conditioning promoted an intense spreading of the cells, while minocycline HCl conditioning induced the formation of a dense feltwork of cellular processes. HPDL fibrolasts adherent to both types of surface‐conditioned dentine exhibited a significantly higher rate of proliferation ( P <0.01) as well as a significantly higher level of total protein and of collagen synthesis ( P <0.01) than on untreated dentine. Conclusions: These data suggest that during periodontal surgery a conditioning of the root surface by citric acid or by minocycline HCl could promote the attachment, the proliferation, and the biosynthetic activity of HDPL, prerequisites to periodontal regeneration. J Periodontol 1999;70:1144‐1152.