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The Utility of Whole Saliva to Detect the Oral Presence of Periodontopathic Bacteria
Author(s) -
Umeda Makoto,
Contreras Adolfo,
Chen Casey,
Bakker Ingalill,
Slots Jørgen
Publication year - 1998
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1998.69.7.828
Subject(s) - treponema denticola , saliva , prevotella intermedia , actinobacillus , mcnemar's test , microbiology and biotechnology , gingival and periodontal pocket , bacteroides , periodontitis , kappa , dentistry , fusobacterium nucleatum , medicine , biology , bacteria , porphyromonas gingivalis , linguistics , statistics , genetics , mathematics , philosophy
T his study compared the presence of 6 periodontopathic bacteria in whole saliva and subgingival plaque of 202 subjects. The test bacteria were identified using a 16S rRNA‐based PCR detection method. Each study subject contributed a whole saliva sample and a paper point sample pooled from the deepest periodontal pocket in each quadrant of the dentition. The kappa test revealed a fair agreement between the presence of Porphyromonas gingivalis, Prevotella intermedia , and Treponema denticola in whole saliva and periodontal pocket samples (kappa > 0.4). The McNemar test showed that the differences between sample types were due to a more frequent detection of the 3 organisms in whole saliva than in periodontal pocket samples ( P < 0.01). Prevotella nigrescens also was detected more frequently in whole saliva than in periodontal pocket samples ( P < 0.01; McNemar test). Although little agreement between samples was found for Actinobacillus actinomycetemcomitans and Bacteroides forsythus (kappa ≤ 0.4), neither whole saliva nor pocket samples showed better detection for these 2 species ( P < 0.01, McNemar test). The results indicate that whole saliva is superior to pooled periodontal pocket samples to detect P. gingivalis, P. intermedia, P. nigrescens , and T. denticola in the oral cavity. The detection of oral A. actinomycetemcomitans and B. forsythus with reasonably good accuracy may require both whole saliva and periodontal pocket samples. J Periodontol 1998;69:828–833 .