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Gingival Epithelial Cells Increase Interleukin‐8 Secretion in Response to Actinobacillus actinomycetemcomitans Challenge
Author(s) -
Huang George T.J.,
Haake Susan Kinder,
Park NoHee
Publication year - 1998
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1998.69.10.1105
Subject(s) - actinobacillus , secretion , microbiology and biotechnology , interleukin 8 , aggregatibacter actinomycetemcomitans , lipopolysaccharide , bacteria , biology , epithelium , pathogen , chemotaxis , extracellular , porphyromonas gingivalis , cell culture , inflammation , chemistry , immunology , biochemistry , genetics , receptor
P eriodontal diseases result from the interaction of bacterial pathogens with the host gingival tissues. The role of gingival epithelial cells in the initiation of host defense mechanisms after encountering oral bacteria has not been investigated. Actinobacillus actinomycetemcomitans is a key periodontal pathogen that adheres to and invades oral epithelial cells. Thus, we examined whether gingival epithelial cells increase secretion of the potent neutrophil chemoattractant interleukin‐8 (IL‐8) following A. actinomycetemcomitans challenge. Normal human oral keratinocytes (NHOK), isolated from gingival tissue, and 3 oral epithelial cell lines (HOK‐18A, HOK‐16B‐BaPT1, and HEp‐2) were co‐cultured with A. actinomycetemcomitans for 2 hours to allow bacteria–epithelial cell interactions. The epithelial cells were then washed, and fresh medium with gentamicin was added to kill extracellular bacteria. Cell cultures were further incubated for 24 hours before the supernatant was collected for IL‐8 detection with ELISA. The results showed that IL‐8 secretion increased 2‐ to 7‐fold 24 hours after bacterial challenge. The highest IL‐8 secretion was at the multiplicity of infection (MOI) of 1,000:1 in bacterial dose response studies using HOK‐16B‐BaP‐T1 cells. Time‐course studies revealed that IL‐8 secretion rapidly reached a maximum level 6 hours after bacterial challenge and subsequently decreased to basal levels. These data indicate that gingival epithelial cells are capable of upregulating IL‐8 expression rapidly in response to A. actinomycetemcomitans challenge and thus may facilitate the recruitment of neutrophils as a host defense mechanism. J Periodontol 1998;69:1105–1110 .