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Increased Proliferation, Collagen, and Fibronectin Production by Hereditary Gingival Fibromatosis Fibroblasts
Author(s) -
Tipton David A.,
Howell Karen J.,
Dabbous Mustafa Kh.
Publication year - 1997
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1997.68.6.524
Subject(s) - fibroblast , fibronectin , extracellular matrix , chemistry , in vitro , type i collagen , microbiology and biotechnology , fibromatosis , extracellular , pathology , biology , biochemistry , medicine
H ereditary gingival fibromatosis (HOF) is a fibrotic enlargement of the gingiva. HGF gingiva contains large amounts of interstitial collagen and other extracellular matrix (ECM) molecules. Increased proliferation and elevated production of the ECM molecules type I collagen and fibronectin (FN) could contribute to the clinical increased bulk of HGF gingiva. Fibroblast‐strains from HGF gingiva and normal human gingival fibroblast strains (GN) were used in this in vitro study. Fibroblast proliferation was determined by ELISA which measured the incorporation of 5‐bromo‐2′‐deoxyuridine into DNA. The results showed that HGF fibroblast strains proliferated more rapidly than GN fibroblasts (68% to 488% increase, depending on the strains) ( P ≥ 0.01), the only exception being one HGF strain versus one normal strain. All HGF strains produced greater amounts of FN (measured by ELISA) than all of the normal fibroblast strains (23% to 49% increase, depending on the strain) ( P ≤ 0.04). Similarly, all HGF strains made significantly greater ( P ≤ 0.03) amounts of type I collagen (also measured by ELISA) than all of the normal strains (55% to 235% increase, depending on the strain). The results show that, in vitro, HGF fibroblasts display several phenotypic characteristics of activated fibroblasts: increased proliferative rates as well as increased production of FN and type I collagen, consistent with in vitro studies of fibroblasts derived from other types of fibrotic tissue. These results suggest that the increased proliferation of HGF fibroblasts and their increased production of extracellular matrix molecules such as collagen and FN may contribute to the clinical gingival enlargement characteristic of HGF. J Periodontol 1997;68:524–530 .