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Comparative Effects of Cyclosporin on Glycosaminoglycan Synthesis by Gingival Fibroblasts
Author(s) -
Newell Jeff,
Irwin Chris R.
Publication year - 1997
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1997.68.5.443
Subject(s) - glycosaminoglycan , connective tissue , pathogenesis , chemistry , hyaluronic acid , cell , fibroblast , glucosamine , microbiology and biotechnology , biology , biochemistry , immunology , medicine , anatomy , pathology , in vitro
H istolotical studies show that drag‐induced gingival overgrowth results from an accumulation of the connective tissue component. Despite comprising a major part of gingival connective tissue, a role for glycosaminoglycans (GAGs) in the pathogenesis of gingival overgrowth has received scant attention. By analyzing the metabolism of 3H‐glucosamine, we have compared GAG and hyaluronan synthesis by fibroblasts derived from normal and overgrown gingival tissue, and the effects of cyclosporin on GAG output. GAG production was cell density‐dependent, fibroblasts cultured at low density synthesizing significantly increased quantities in comparison to confluent cultures. The effects of cyclosporin on GAG synthesis was also found to be both cell density‐ and cell strain‐dependent. However, cyclosporin‐stimulated GAG synthesis by 2/3 overgrown cell strains and 1/3 normal strains. These results suggest that a direct promotion of GAG synthesis by gingival fibroblasts in response to cyclosporin may play a role in the pathogenesis of gingival overgrowth. J Periodontol 1997;68:443–447 .