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Oral Food Consumption and Subgingival Microorganisms: Subgingival Microbiota of Gastrostomy Tube‐Fed Children and Healthy Controls
Author(s) -
Chen Casey,
Ashimoto Atsushi,
Sangsurasak Sumalee,
Flynn M. Jane,
Slots Jørgen
Publication year - 1997
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1997.68.12.1163
Subject(s) - prevotella intermedia , microbiology and biotechnology , fusobacterium , eikenella corrodens , actinomyces , biology , actinobacillus , treponema denticola , capnocytophaga , bacteroides , prevotella , oral microbiology , veillonella , porphyromonas gingivalis , periodontitis , medicine , dentistry , bacteria , streptococcus , genetics
T his study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube‐fed children and 24 healthy controls. Microbial identification was carried out using anaerobic culture and 16S rRNA‐based PCR identification methods. Streptococcal and Actinomyces species were recovered from 100% and 76% of all subjects and averaged 66% and 11% of total cultivable organisms, respectively. In decreasing order of prevalence, Fusobacterium , enteric rods, Prevotella intermedia/Prevotella nigrescens, Capnocytophaga, Propionibacterium , yeasts, Actinobacillus actinomycetemcomitans , coagulase‐negative Staphylococcus, Campylobacter rectus, Bacteroides forsythus , and Porphyromonas gingivalis were detected in 48% to 2% of the study subjects. The cultivable levels of these species varied widely among subjects. PCR detection showed C. rectus and Eikenella corrodens both to occur in 93% of the study subjects and to be the most prevalent putative periodontal pathogens examined. In decreasing order of prevalence, PCR identified Treponema denticola, A. actinomycetemcomitans, P. nigrescens, P. intermedia, B. forsythus, and P. gingivalis in 38% to 21% of the subjects studied. Tube‐fed children and healthy controls exhibited similar subgingival microbial compositions. It appears from this study that oral food consumption is not a major determinant for the establishment of subgingival microbiota in children. J Periodontol 1997;68:1163–1168.