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Alveolar Bone Loss in Rats Infected With a Strain of Prevotella intermedia and Fusobacterium nucleatum Isolated From a Child With Prepubertal Periodontitis
Author(s) -
YoshidaMinami Ichie,
Suzuki Atsuko,
Kawabata Keiko,
Okamoto Akiko,
Nishihara Yumi,
Minami Takahiro,
Nagashima Shigeru,
Morisaki Ichijiro,
Ooshima Takashi
Publication year - 1997
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1997.68.1.12
Subject(s) - prevotella intermedia , fusobacterium nucleatum , dental alveolus , periodontitis , microbiology and biotechnology , bacteroidaceae , porphyromonas gingivalis , medicine , periodontal pathogen , biology , bacteria , dentistry , genetics
P revotella intermedia and F usobacterium nucleatum are associated with various forms of periodontal disease. The purpose of the present study was to infect the clinical isolates of these periodontopathic bacteria and to induce a significant loss of alveolar bone in specific pathogen‐free (SPF) rats in the absence of ligatures. P. intermedia YKD8 and F. nucleatum YKZ5 were isolated from a prepubertal periodontitis patient, while P. gingivalis MWB13 was from a patient with juvenile periodontitis. At first, SPF Sprague‐Dawley rats (70 days of age, male) were infected with A. viscosus NylR and subsequently superinfected with P. gingivalis MWB13, P. intermedia YKD8, or F. nucleatum YKZ5, respectively. The control group was infected with A. viscosus Ny1R alone. All rats were killed and periodontal bone levels were assessed morphometrically 135 days after the first infection with A. viscosus. P. intermedia YKD8 was recovered frequently from rats, with serum antibody levels remaining highly elevated throughout the experiment. Significant loss of alveolar bone was found in rats infected with P. intermedia YKD8, the virulence of which was equivalent to that of P. gingivalis MWB13. F. nucleatum YKZ5 also induced alveolar bone loss, but not significantly when compared with rats infected with A. viscosus Ny1R alone. J Periodontol 1997;68:12–17 .