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HLA Class II Genotypes Associated With Early‐Onset Periodontitis: DQB1 Molecule Primarily Confers Susceptibility to the Disease
Author(s) -
Ohyama Hideki,
Takashiba Shogo,
Oyaizu Kosuke,
Nagai Atsushi,
Naruse Taeko,
Inoko Hidetoshi,
Kurihara Hidemi,
Murayama Yoji
Publication year - 1996
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1996.67.9.888
Subject(s) - human leukocyte antigen , genotype , periodontitis , disease , periodontal disease , genetics , allele , medicine , biology , immunology , gene , dentistry , antigen
DNA typing was performed on 24 Japanese patients with early‐onset periodontitis (EOP) using the PCR‐RFLP method to investigate an association of the susceptibility to EOP with the particular HLA class II alleles (HLA‐DRB1, ‐DQA1, and ‐DQB1). DRB1* 1401, DRB1* 1501, DQB1* 0503, and DQB1* 0602 were found more frequently (“susceptible”) in the EOP patients than in healthy controls. In contrast, DRB1* 0405 and DQB1* 0401 were found less frequently (“resistant”) in EOP patients. All patients carrying DQB1* 0602 had an atypical Bam HI site in the intron upstream of the third exon of the DQB1 gene, which in our previous studies appeared to be a susceptible marker for EOP. A comparative analysis of the amino acid sequences of these susceptible and resistant HLA‐DRB1 and DQB1 alleles elucidated some differences in antigen‐derived peptide binding sites related to the susceptible or resistant alleles. Especially, DQB1* 0503 and DQB1* 0602 alleles carrying aspartic acid at position 57 and glycine at position 70 are increased significantly in EOP. Since amino acid residues at positions 57 and 70 on the DQB1 molecule are supposed to be involved in antigen binding, amino acid substitutions at these positions may affect the immune responsiveness to the periodontopathic antigen. Our results suggest that the DQB1 molecule plays a crucial role in the pathogenesis of EOP and that the susceptibility to EOP may be determined by the binding ability between the peptide and HLA‐DQ antigens. J Periodontol 1996;67:888–894 .