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Aspartate Aminotransferase (AST) Levels in Human Periodontium‐Derived Cells
Author(s) -
Mizuho Fuyuki,
Mori Hiroshi,
Deguchi Shinji,
Ogawa Yuji,
Hori Toshio
Publication year - 1996
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1996.67.8.733
Subject(s) - periodontal fiber , periodontium , periodontal disease , in vitro , medicine , chemistry , endocrinology , pathology , dentistry , biochemistry
T he ability to objectively assess periodontal disease activity can significantly affect periodontal therapy. Aspartate aminotransferase (AST) is released extracellularly upon tissue destruction which suggests its potential as a key index in a quantitative assay for evaluating periodontal disease activity. The purpose of the present research was to assess the origin of AST in gingival crevicular fluid (GCF) in vitro. An experimental kit was used for the measurement of AST level in human gingival epithelial cells (HGEs), human gingival fibroblasts (HGFs), human periodontal ligament fibroblasts (HPLFs), polymorphonuclear leukocytes (PMNs), and plasma in peripheral blood. AST activify levels were observed in all of the periodontally derived cells, PMNs, and plasma. A significantly high level of AST activity was observed in HGEs (104.93 ± 8.13 KU/1000 cells). The level of AST activity in HPLFs was 18.09 ± 3.73 KU/1000 cells. AST activity in PMNs was significantly low, approximately 2% of that observed in HPLFs. These findings may suggest that AST level in GCF is correlated with the destruction of periodontal tissue. J Periodontol 1996;67:733–736 .