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PDGF‐B Producing Cells and PDGF‐B Gene Expression in Normal Gingiva and Cyclosporine A‐Induced Gingival Overgrowth
Author(s) -
Plemons Jacqueline M.,
Dill Russell E.,
Rees Terry D.,
Dyer Barbara J.,
Ng May C.,
Iacopino Anthony M.
Publication year - 1996
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1996.67.3.264
Subject(s) - platelet derived growth factor receptor , dentistry , medicine , chemistry , growth factor , receptor
I t has been proposed that healthy gingiva is in a continuous state of wound repair. Thus, one might expect to find cells in normal gingiva producing growth factors associated with wound healing such as platelet‐derived growth factor B chain (PDGFB). One might also expect to find increased numbers of these cells or increased amounts of these growth factors in conditions which involve increased tissue volume such as drug‐induced gingival overgrowth (DGO). The purpose of this study was to quantify PDGF‐B gene expression and identify cells producing PDGF‐B in normal gingiva and DGO. Cyclosporine A (CSA) DGO was selected as a prototype of the overgrowth condition. Twelve patients with clinical CSA DGO and 12 patients with no DGO or history of drugs known to cause DGO were selected for study. Frozen sections of gingival specimens from these patients were subjected to in situ hybridization for PDGF‐B mRNA. Positive cells were counted and expressed as mean ± SEM cells/mm 2 of lamina propria. Morphometric analysis revealed 6.2 ± 1.9 cells/mm 2 for control gingiva and 10.3 ± 3.4 cells/mm 2 for CSA DGO samples. There was no statistically significant difference between groups. PDGF‐B gene expression was measured in these cells and expressed as mean ± SEM silver grains/cell. There was a significant upregulation of PDGF‐B gene expression in cells from the CSA DGO group (39.5 ± 14.7 silver grains/cell for normal gingiva vs. 255.3 ± 77.1 silver grains/cell for CSA DGO samples; P < 0.001). The presence of PDGF‐B in these cells was confirmed in all cases by immunocytochemical localization. Additionally, PDGF‐B producing cells were identified as macrophages in sections taken from an additional patient with CSA DGO by double immunofluorescence labeling of the CD51 membrane marker for macrophages and intracellular PDGF‐B. These findings are consistent with the concept that healthy gingiva is in a continuous state of wound repair and support the hypothesis that CSA DGO is associated with enhanced macrophage PDGFB gene expression rather than an increase in the number of PDGF‐B producing macrophages. J Periodontol 1996;67:264–270 .