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Increased Expression of Interleukin‐1 Receptors on Fibroblasts Derived From Inflamed Gingiva
Author(s) -
KandaNakamura Chieko,
Izumi Yuichi,
Sueda Takeshi
Publication year - 1996
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1996.67.12.1267
Subject(s) - fibroblast , immunohistochemistry , monoclonal antibody , receptor , interleukin , inflammation , staining , chemistry , pathology , medicine , microbiology and biotechnology , immunology , cytokine , antibody , cell culture , biology , genetics
W e investigated the expression of interleukin‐1 receptors (IL‐1R) on the surfaces of cultured gingival fibroblasts derived from healthy and inflamed gingiva and the effects of IL‐1α and IL‐1β and prostaglandin E 2 (PGE 2 ) on IL‐1R expression. Fibroblasts were obtained from expiant culture of both healthy and inflamed gingiva. IL‐1R on cell surfaces was detected immunohistochemically using an anti‐human IL‐1R monoclonal antibody. IL‐1R expression was assessed quantitatively using an enzyme linked immunosorbent assay (ELISA). Positive staining for IL‐1R was more evident on cells from inflamed gingiva compared with cells from healthy gingiva. The ELISA showed a significantly increased number of IL‐1R on cells from inflamed gingiva compared with cells from healthy gingiva ( P < 0.05). Treatment with IL‐1 but not PGE 2 increased expression of IL‐1R on fibroblasts. These findings suggest that gingival fibroblast responses to IL‐1 may represent a mechanism for amplification of gingival inflammation. J Periodontol 1996;67:1267–1273 .