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Metal Substrates Influence the Release of Glycosaminoglycan and Transforming Growth Factor β by Human Bone Cells
Author(s) -
Locci Paola,
Becchetti Ennio,
Pugliese Mariano,
Rossi Leonardo,
Lilli Cinzia,
Calvitti Mario,
Staffolani Nicola
Publication year - 1996
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1996.67.12.1260
Subject(s) - glycosaminoglycan , chemistry , transforming growth factor , microbiology and biotechnology , growth factor , metal , biophysics , biochemistry , biology , receptor , organic chemistry
B one cells derived from human jaw were isolated from expiants and grown in vitro. Subcultures were cultured on plastic (control) and metal substrates for 24 and 48 hours in medium containing 3 H‐glucosamine and labeled glycosaminoglycan (GAG) accumulation was measured. In bone cells cultured on metal substrates there was an evident reduction in the synthesis and secretion of radiolabeled macromolecules compared to bone cells‐cultured on plastic. Moreover, the accumulation of single GAG classes was specific for each substrate tested. The results showed that titanium was the only metal substrate studied in which the percentage of individual GAG classes remained the same as control cultures. GAG reduction was due to a decreased synthesis and not to an increased degradation as shown by the decrement of exoglycosidase activity. The metals also reduced the activity of transforming growth factor β (TGFβ), measured using interleukin‐1 assay method, a factor involved in the various phases of bone remodeling; in this case, too, cells grown on titanium showed the highest TGFβ activity compared to the other metal substrates studied. The results indicate that the substrate to which the cells adhere do exhibit specific differences in GAG composition and TGFβ activity. The differences observed may be important during in vivo events such as guided tissue regeneration and bone deposition. J Periodontol 1996;67:1260–1266 .