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Interleukin‐1β Gene Expression in Human Oral Polymorphonuclear Leukocytes
Author(s) -
Hendley Thomas M.,
Steed R. Britt,
Galbraith Gillian M.P.
Publication year - 1995
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1995.66.9.761
Subject(s) - gene expression , gene , immunology , medicine , biology , genetics
O ral polymorphonuclear leukocytes ( pmn ) were obtained from 10 adult donors in good oral health using a method employing repeated mouth rinse collection. Interleukin‐1β (IL‐1β) mRNA was detected in freshly obtained cells by blot hybridization of total cellular RNA with a biotin labeled cDNA probe. Supernates from oral PMN placed in culture for 3 hours contained substantial amounts of IL‐1β measured by ELISA. Significantly greater numbers of PMN and amounts of PMN‐derived IL‐1β were obtained from the same donors 2 hours subsequent to an oral sucrose challenge (3.23 × 106 vs. 1.57 × 106 mean PMN number, P = 0.004; 59.80 vs. 20.05 mean pg/ml IL‐1β, P = 0.036, respectively). However, the elevated levels of IL‐1β were due to the higher cell number rather than to increased production by individual cells. Stimulation of oral PMN with recombinant granulocyte‐macrophage colony stimulating factor did not enhance their cytokine production. In most instances, IL‐1β production by oral PMN was dramatically greater than that of their blood counterparts. These findings suggest that oral PMN are an important source of IL‐1 β , which plays a central role in oral immunity and inflammatory disease states. J Periodontol 1995; 66:761–765 .