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The Effects of Scaling Procedures on Epithelial Cell Growth on Titanium Surfaces
Author(s) -
Kuempel Deena R.,
Johnson Georgia K.,
Zaharias Rebecca S.,
Keller John C.
Publication year - 1995
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1995.66.3.228
Subject(s) - titanium , materials science , scanning electron microscope , biomedical engineering , implant , cell growth , chemistry , metallurgy , composite material , surgery , medicine , biochemistry
T his study examined in vitro epithelial cell growth on titanium surfaces which had been instrumented by 3 types of periodontal curets. Commercially pure titanium discs were uniformly scaled using plastic, stainless steel, or gold‐coated curets and then seeded with a microdot (50,000 cells per 10 μl) of rat gingival epithelial cells. After 5 days of growth, epithelial cell surface area (mm 2 ) coverage was measured on photographed specimens using a computer digitizing system. Scanning electron microscopic evaluation showed a denser central core of cellular growth on the stainless steel‐treated surfaces as compared to the other surface treatments. Epithelial surface area coverage on the stainless steel, plastic, and control groups did not vary significantly among groups. However the gold‐coated curet exposed surfaces supported significantly less epithelial growth than the stainless steel and control surfaces. A better understanding of in vitro epithelial cell responses to different titanium surface characteristics will assist in designing implant scaling procedures which allow maintenance of the implant perimucosal seal. J Periodontol 1995; 66:228–234 .