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A Comparison of the Effect of Epidermal Growth Factor, Platelet‐Derived Growth Factor, and Fibroblast Growth Factor on Rat Periodontal Ligament Fibroblast‐Like Cells' DNA Synthesis and Morphology
Author(s) -
Blom Søren,
Holmstrup Palle,
Dabelsteen Erik
Publication year - 1994
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1994.65.5.373
Subject(s) - periodontal fiber , fibroblast , epidermal growth factor , fibroblast growth factor receptor 2 , microbiology and biotechnology , basic fibroblast growth factor , growth factor , fibroblast growth factor receptor 3 , fibroblast growth factor , biology , dna synthesis , cell growth , platelet derived growth factor , wound healing , cell culture , platelet derived growth factor receptor , chemistry , immunology , in vitro , biochemistry , genetics , dentistry , medicine , receptor
A n enhanced formation of bone, dentin, and collagen fibers in periodontal wounds after application of polypeptide growth factors has recently been reported. However, the complex environment in vivo makes it impossible to determine the specific effects of growth factors on various cells involved in the wound‐healing process. We have therefore investigated the mitogenic and morphogenic effects of recombinant epidermal growth factor (rEGF), natural platelet‐derived growth factor (nPDGF), and natural fibroblast growth factor (nFGF) on periodontal ligament fibroblast‐like cells. A cell line was established from rat PDL tissue. The cell line was characterized according to morphology, growth pattern, cytoskeletal proteins, and growth kinetics. The mitogenic effect of growth factors was assessed by incorporation of [ 3 H]thymidine in the cellular DNA for 4 hours. Differences between groups of observations were assessed by the Student t ‐test. The morphogenic effects of growth factors were described with respect to growth pattern, cell orientation, and cell and nucleus form after a random photographic recording. The fibroblast‐like cell type and the non‐transformed phenotype of the cell line have been identified by the presence of parameters considered to be characteristic of a normal fibroblast‐like cell line. The morphogenic analysis of both experimental and control cultures showed a monolayer of adherent cells with spindle or stellate morphology, a random alignment and round or elongated nuclei. Incorporation of [ 3 H]‐thymidine was increased in a dose‐dependent manner by all growth factors. Maximal effect on the DNA synthesis was: rEGF, 131%; nPDGF, 274%; and nFGF, 182%. The results show that growth factors in high concentrations have no dramatic effects on the morphology of PDL fibroblast‐like cells. However, variation in the mitogenic potency of the growth factors should be considered when these growth factors are used in periodontal treatments in the future. J Periodontol 1994; 65:373–378.