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Immunohistochemical Analysis of Tissues Regenerated From Within Periodontal Defects Treated With Expanded Polytetrafluoroethylene Membranes
Author(s) -
PritloveCarson Sandra,
Palmer Richard M.,
Floyd Peter D.,
Morgan Peter M.
Publication year - 1994
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1994.65.2.134
Subject(s) - vimentin , immunohistochemistry , regeneration (biology) , membrane , pathology , epithelium , mesenchymal stem cell , soft tissue , staining , chemistry , wound healing , mucous membrane , monoclonal antibody , anatomy , medicine , biology , microbiology and biotechnology , antibody , surgery , biochemistry , immunology
I mmunocytochemical analysis was carried out on samples of 5‐, 6‐, and 9‐week old regenerated soft tissue taken from healing periodontal defects treated by guided tissue regeneration using expanded polytetrafluorethylene (ePTFE) membranes. A panel of monoclonal and polyconal antibodies to cytokeratins, vimentin, and collagen was used to label cells and collagen types I, III, and IV. Epithelium was identified in 7 of the 9 samples examined, in addition to mesenchymal cells staining positively for vimentin and co‐distribution of collagen types I, III, and IV in all samples. Clinical observations indicated that exposure of the ePTFE membranes during healing was a frequent occurrence, and the presence and quantity of epithelium found within the healing defect beneath the membrane may be related to the extent to which this occurs. J Periodontol 1994;65:134–138 .