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A Cross‐Sectional Study on Osteocalcin Levels in Gingival Crevicular Fluid From Periodontal Patients
Author(s) -
Kunimatsu Kazushi,
Mataki Shiro,
Tanaka Hidetaka,
Mine Naoko,
Kiyoki Mamoru,
Hosoda Kenji,
Kato Yuzo,
Kato Ihachi
Publication year - 1993
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1993.64.9.865
Subject(s) - osteocalcin , gingivitis , periodontitis , medicine , dentistry , clinical attachment loss , bleeding on probing , chronic periodontitis , interdental consonant , endocrinology , gastroenterology , chemistry , alkaline phosphatase , biochemistry , enzyme
T he purpose of the present study was to determine the levels of osteocalcin, a bone specific matrix protein, in gingival crevicular fluid (GCF) from periodontal disease patients and to investigate the relationship between GCF osteocalcin levels and clinical parameters. Nineteen initial visit patients, 5 patients with gingivitis and 14 patients with adult Periodontitis, participated in this study. The clinical parameters including probing depth, attachment level, gingival index, and tooth mobility were recorded following careful sampling of GCF with a filter paper strip harvested for 3 minutes. Osteocalcin adsorbed on a strip was extracted in a plastic tube containing 150μ1 of 10 mM sodium phosphate buffer (pH 6.5). GCF osteocalcin was determined by a newly‐developed, high sensitive enzyme immunoassay which could recognize the N‐terminal 20 residue peptide. In gingivitis patients, no significant amounts of osteocalcin were detected. In Periodontitis patients, on the other hand, osteocalcin levels were detected, ranging between 0 and 540 pg/tube and positively correlated with these clinical parameters ( P <0.01). Moreover, in several sites in GI = 3 group, extremely higher levels of GCF osteocalcin were detected. These results strongly suggest that in addition to the presence of GCF osteocalcin the levels of osteocalcin may reflect the degree of the periodontal inflammation at the sampled sites. J Periodontol 1993; 64:865–869.