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Mitogenic Effects of Growth Factors on Human Periodontal Ligament Cells In Vitro
Author(s) -
Oates Thomas W.,
Rouse Cheryl A.,
Cochran David L.
Publication year - 1993
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1993.64.2.142
Subject(s) - periodontal fiber , platelet derived growth factor receptor , cell growth , dna synthesis , in vitro , cell culture , growth factor , endocrinology , platelet derived growth factor , thymidine , medicine , beta (programming language) , chemistry , biology , biochemistry , dentistry , receptor , genetics , computer science , programming language
P eriodontal regeneration is thought to require the migration and proliferation of periodontal ligament cells. Evidence suggests that the Polypeptide growth factors PDGF, IL‐1, and TGF‐β are mediators of these cellular events in wound healing. The purpose of this study was to determine the effects of these growth factors on human periodontal ligament (PDL) cell mitogenesis, and to identify the regulatory influences of TGF‐β on the response to PDGF and IL‐1. Confluent, quiescent human PDL cells were cultured in vitro and treated with the Polypeptide growth factors PDGF‐AA and ‐BB, IL‐1β, and TGF‐• in both a dose and time‐dependent manner. Mitogenic activity, as a measure of proliferative potential, was determined by the quantitation of 3 H‐thymidine incorporation during DNA synthesis. The results of this study demonstrated that both PDGF‐AA and ‐BB enhance mitogenic activity in a dose‐dependent manner over a concentration range of 1.0 to 50.0 ng/ml. IL‐1β (0.01 to 1.0 pM) resulted in no mitogenic enhancement, and at high concentrations (10.0 to 100.0 pM) demonstrated an inhibitory effect. TGFβ produced a significant increase ( P <0.01) in mitogenic activity (although relatively much less than PDGF) in a delayed, bimodal, dose‐dependent manner over a concentration range of 0.01 to 20.0 ng/ml, with a maximal response at a concentration of 1.0 ng/ ml. Additionally, incubation with TGF‐β at 1.0 ng/ml prior to the addition of PDGF significantly enhanced ( P <0.01) the mitogenic response to both PDGF‐AA and PDGFBB. In contrast, incubation with TGF‐β at a higher concentration (10.0 ng/ml) prior to PDGF addition resulted in a decreased ( P <0.05) mitogenic response to PDGF‐AA, while enhancing ( P <.01) the response to PDGF‐BB. This study demonstrates that PDGF‐AA and PDGF‐BB are major mitogens for human PDL cells in vitro, and supports a role for TGF‐β as a regulator of the mitogenic response to PDGF in these cells. J Periodontol 1993; 64:142–148 .