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The Influence of Doxycycline on the Attachment of Fibroblasts to Gelatin‐Coated Surfaces and Its Cytotoxicity
Author(s) -
Tsukuda Nobukazu,
Gabler Walter L.
Publication year - 1993
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1993.64.12.1219
Subject(s) - cytotoxicity , propidium iodide , cytotoxic t cell , tetracycline , chemistry , fibroblast , doxycycline , gelatin , lactate dehydrogenase , microbiology and biotechnology , periodontal fiber , neutral red , formazan , pharmacology , apoptosis , biochemistry , medicine , biology , antibiotics , in vitro , dentistry , programmed cell death , enzyme
B ecause of their antimicrobial and anti‐inflammatory properties, tetracyclines have been used systemically and locally in the treatment of periodontal disease. This study was done to evaluate the influence of doxycycline (De), a tetracycline, on fibroblast adherence to a protein coated surface and its cytotoxicity. Periodontal ligament derived fibroblasts (PLDFs) were either: 1) preincubated with Dc (0 to 100 μg/ml) and then allowed to adhere to a gelatin‐coated surface or 2) adherence was first established and then Dc added to the system. After an appropriate time the number of PLDFs adherent, released, or lysed was estimated by a lactic dehydrogenase (LDH) assay. Preincubation of PLDFs in 25 μg Dc/ml or higher concentrations significantly ( P <0.01) reduced the number of adherent cells. Fifty μg Dc/ml and higher doses significantly ( P <0.01) increased PLDFs cytotoxicity as measured by LDH release. The same trend was noted if PLDFs were allowed to adhere and then subjected to the drug. Microscopic observation of fluorescein diacetate/propidium iodide‐stained cells showed that attached‐spread cells pulled in, rounded up, and detached in proportion to the increased dose of Dc and the percentage of red‐stained cytotoxic cells rose in a similar manner. The data suggested that Dc can be cytotoxic and may inhibit PLDFs adherence and spread along a substratum. J Periodontol 1993;64:1219–1224 .