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Root Dentin Morphology After Different Modes of Citric Acid and Tetracycline Hydrochloride Conditioning
Author(s) -
Labahn Roland,
Fahrenbach Wolf H.,
Clark Stephen M.,
Lie Tryggve,
Adams Donald F.
Publication year - 1992
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1992.63.4.303
Subject(s) - tetracycline hydrochloride , citric acid , dentin , chemistry , tetracycline , dentistry , conditioning , pharmacology , biology , medicine , biochemistry , mathematics , antibiotics , statistics
T he purpose of this study was to assess the effect of citric acid and tetracycline HCl application to dentin surfaces by a “passive dripping” or an “active burnishing” technique. Twenty dentin blocks were prepared from freshly extracted non‐diseased human impacted third molars. The blocks were root planed and randomly assigned to two groups for treatment with either citric acid or tetracycline HCl. The duration of treatment was 30, 60, 120, or 240 seconds. Control blocks were treated with distilled water. After treatment the blocks were processed for observation and measurements in the scanning electron microscope (SEM). Application of either of the acid solutions resulted in removal of the smear layer. Measurements indicated a time dependent increase in the mean dentinal tubule orifice diameter ranging from 1.05 μm in control specimens to 3.18 μm after 4 minutes treatment (citric acid group). The increase in tubule diameter was significantly greater ( P ≤ 0.01) for both citric acid treatment modalities than tetracycline HCl treatment. There was also a time dependent increase in the depth of penetration as measured by a trumpeting of the tubule profiles, and this penetration was significantly greater ( P ≤ 0.01) after citric acid treatments. Passive or active application of the acids did not seem to have any major impact on the measurements or on the surface morphology. It was concluded that citric acid causes more extensive changes than tetracycline HCl and that the mode of application of the agent is probably not critical. J Periodontol 1992; 63:303‐309 .