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Immunohistochemical Analysis of Cells Attached to Teflon Membranes Following Guided Tissue Regeneration
Author(s) -
PritloveCarson Sandra,
Palmer Richard M.,
Morgan Peter R.,
Floyd Peter D.
Publication year - 1992
Publication title -
journal of periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.036
H-Index - 156
eISSN - 1943-3670
pISSN - 0022-3492
DOI - 10.1902/jop.1992.63.12.969
Subject(s) - regeneration (biology) , immunohistochemistry , membrane , microbiology and biotechnology , chemistry , pathology , biomedical engineering , anatomy , medicine , biology , biochemistry
A wide panel of monoclonal antibodies to various keratins together with an antivimentin antibody to label mesenchymal cells were used to label cells attached to expanded polytetrafluorethylene (ePTFE) membranes removed 6 weeks postoperatively from 7 patients. All membranes demonstrated variable amounts of adherent tissue, within which vimentin‐positive cells were detected. In 8 of the 18 samples, no epithelial cells were detected. Seven of the 18 samples demonstrated small groups of epithelial cells on both sides of the membranes, staining predominantly as basal cells. The remaining 3 samples were more densely populated by epithelium which expressed a diverse keratin profile. Thus, the membranes were successful in establishing mesenchymal cell proliferation, as evidenced by vimentin positivity, but did not totally exclude epithelium. J Periodontol 1992; 63:969–973.